The U.S.-Singapore Third Country Training Program (TCTP) will host a Molecular Epidemiology workshop with in-person training sessions from December 2 to 6, 2024, in Singapore. The course seeks to equip participants working in the tuberculosis field with a comprehensive understanding of next generation sequencing (NGS) technologies, in particular whole genome sequencing (WGS), and targeted NGS (tNGS) as integral tools for tuberculosis control and outbreak investigations.